retroviral

Embryonic stem cells use ZFP809 to silence retroviral DNAs.

胚胎干细胞利用ZFP809使逆转录病毒DNA沉默。

Construction of the recombinent retroviral vector rRV-hIL-4 and expression of rheumatoid arthritis in vitro.

携带hIL-4逆转录病毒重组体的构建及在类风湿关节炎中体外表达的研究

The safety assessments on retroviral mediated HSV tk/ACV gene therapy for glioma have been made.

研究了反转录病毒介导的HSV－tk/ACV系统基因治疗脑肿瘤的安全性。

After A549 was infected by recombinant retroviral vector expressing antisense ANG,ANG protein level decreased greatly.

构建出含反义血管生成素的逆转录病毒载体 ,感染A5 4 9细胞后 ,能有效抑制A5 4 9细胞血管生成素蛋白的表达。

Here, we review the advance in the research of retroviral vector in the hemophilia A gene therapy.

本文对逆转录病毒在血友病A基因治疗中的研究进展作一综述。

Two types of vectors are commonly used, each of which can be introduced by electroporation or retroviral infection.

通常采用外显子陷阱和内含了陷阱两类。

The retroviral genetransfer system is nicely fit for the ex vivo gene therapy for mesangio-proliferative glomerular disease.

结论：该逆转录病毒载体基因转移系统能很好地适用于系膜增殖性肾小球疾病的间接体内基因治疗研究。

The 3FLAG-Id3 fusion gene was amplified from p3FLAG-Id3-CMV-10 vector and subcloned into retroviral vector pMX-CITE/IRES-EGFP.

以p3FLAG-Id3-CMV-10为模板,用PCR扩增3FLAG-Id3融合基因,将其亚克隆至逆转录病毒载体pMX-C ITE/IRES-EGFP中;

Human immunodeficiency virus type 1 integrase is an essential enzyme for retroviral replication and the rational target of anti AIDS drugs.

1整合酶是逆转录病毒复制所必要的酶,因此也是抗艾滋病药物作用的一个合理靶点。

In lupus patients in such large number of virus replication retroviral sequence, they will have such an antibody enzyme.

而在狼疮病人体内，这种逆转录序列病毒大量复制，因此他们才会产生这种抗体酶。

Recombinant retroviral vector expressing antisense ANG can efficiently inhibit ANG expression in A549 cell in vitro,providing the basis for further study in vivo.

利用反义血管生成素的逆转录病毒载体能够有效抑制培养细胞血管生成素的表达 ,为今后的体内应用奠定了基础。

Intravenous in vivo transfection of retroviral vector bearing hRI sequence suppressed the growth of B16 melanoma inoculated in C57BL mice through antiangiogenesis mechanism.

介导hRI基因的逆转录病毒载体可以经静脉在小鼠体内转染通过抗血管生成实现对B16黑色素瘤的抑制。 hRI在肿瘤纤维包膜中的成纤维细胞的高表达提示肿瘤发生时快速生长的成纤维细胞可以成为重要靶点。

Objective To construct recombinant retroviral vector expressing antisense ANG and transfect it into the human lung cancer cell line A549 to inhibit the expression of ANG.

目的 构建含反义人血管生成素的真核表达载体 ,并转染人肺癌细胞株A5 4 9,使之特异性地封闭A5 4 9细胞中血管生成素的表达 ,以达到抑制肿瘤生长的目的。

Using a defective retroviral vector, the investigators transduced ex vivo CD34+ bone marrow cells from five boys with this SCID, resulting in no adverse effects.

调查者使用一种有缺陷的逆转录病毒载体，把5名重症联合免疫缺陷患儿的CD34+骨髓细胞取出体外并进行转换。

Retrovirology . 2004 Sep 28;1(1):30.Gao, G .，Guo，X and Goff, S.P. 2002.Inhibition of Retroviral RNA Production by ZAP, a CCCH-type Antiviral Protein.Science 297:1703-1706.

ZAP抗病毒机制的研究不仅可能为病毒的防治提供新的策略和技术手段，也将有助于我们更深入了解RNA稳定性的调控机理。

Here we introduce the composition of this system and the mechanism of virus transduction and summarize the application and prospect of retroviral expression system.

本文对逆转录病毒表达系统的组成、感染的特点和机制及其应用前景作了概述。

Clearly, this retroviral gene fills an important niche in mammalian biology, but, on the other hand, mammals were able to reproduce perfectly well before they were first infected.

显然，这种逆转录病毒基因填补了哺乳动物生物学的一个小生境，但是从另一方面来说，在第一次感染此类病毒以前，哺乳动物已经能够完美的生殖了。

As a result of retroviral representatives, HTLV apparent destruction of the human immune system, they envisaged, the retrovirus should be the culprits.

由于作为逆转录病毒的代表，HTLV明显的摧毁人的免疫系统，因此他们设想，逆转录病毒应当是罪魁祸首。

Exogenous hIL 10 gene can be transferred into Rat CMC and be expressed stably. The retroviral vector PLX(hIL 10)SN shall lay foundation for researching hIL 10 mechanism and its application for curing heart diseases.

外源性hIL?10基因可以转移到大鼠心肌细胞并稳定表达，为今后研究hIL?10的作用机制及在治疗疾病中的应用打下了基础。

AIM: It is to investigate that apoptosis of MDA-MB-435 cell, a human breast cancer cell line, was induced by recombinant retroviral vector mediated CED-4 gene of cadherin 5 N terminal and its apoptosis mechanism.

目的:研究粘附素5 N 端CED-4(cadherin extracellular domain-4)基因经重组逆转录病毒介导转染人乳腺癌细胞MDA-MB-435 及其诱导MDA-MB-435 细胞凋亡的分子调控机制。

The endogenous retroviral NP9 gene was isolated and cloned using reverse transcription polymerase chain reaction (RT-PCR) and T-A cloning techniques, and its sequence was determined with Perkin-Elmer ABI 377 DNA Sequencer.

对NPg基因编码的蛋白质序列分析结果显示:NPg表达的蛋白质是一种强碱性蛋白质，其等电点为9.59，其中精氨酸占13.5%(l 0/74aa)。

Methods 23 patients with HIV/AIDS(7 HIV,16 AIDS)were epidemically investigated and clinically observed. All the patients with AIDS were treated against opportunity infection,9 patients were treated with high active anti retroviral therapy (HAART).

方法 :对 2 3例艾滋病病毒 (HIV)感染及AIDS病人 (其中HIV感染 7例 ,AIDS 16例 )进行流行病学调查和临床观察 ,16例AIDS病人均给予抗机会性感染治疗 ,9例应用高效抗逆转录病毒治疗 (HAART)。

The vectors which are used in gene therapy include adenoviral (Ad) vectors, retroviral (RV) vectors, adeno-associated virus (AAV) vectors, herpes simplex virus (HSV) vectors and hybrid viral vector.

目前介导基因转移的病毒载体包括腺病毒(adenoviral，Ad)载体、逆转录病毒(retroviral，Rv)载体、腺相关病毒(adeno一assoeiated virus，AAv)载体、单纯疙疹病毒(herpes simPlex virus，Hsv)载体以及多种形式的嵌合病毒载体等。

Research conducted at Tulane University Medical Center suggests that Non-HIV AIDS is associated with a retroviral particle called Human Intracisternal A-Type Particle-Type II,or HIAP-II.

在美国杜兰大学医学中心进行的研究表明，非艾滋病毒艾滋病与一个叫做人类A型粒子-II型，或协二的逆转录病毒颗粒有关。

Methods Green fluo rescence protein(GFP)gene was used as a reporter gene,and was transferred int o lens epithelial cells mediated by retroviral vector,GFP expression in cells w as analyzed.

方法用绿色荧光蛋白基因作为报告基因,以逆转录病毒载体携之转染晶状体上皮细胞,观察GFP在晶状体上皮细胞的表达情况。

"This has significance for Gombe chimp health and park management, disease ecology as it relates to retroviral emergence, and to ape conservation as a whole by using Gombe as a laboratory.

“这具有重要意义的贡贝黑猩猩健康和公园管理，疾病生态，因为它关系到逆转录病毒出现，并人猿保护作为一个整体使用贡贝作为一个实验室。

In some virus in the self-replicating RNA (such as etc.) and, in some viruses can retroviral RNA as a template DNA into the process (of some carcinogenic virus) is the law of the Centre added.

在某些病毒中的RNA自我复制（如烟草花叶病毒等）和在某些病毒中能以RNA为模板逆转录成DNA的过程（某些致癌病毒）是对中心法则的补充。

The retroviral vector (RCAS) has been widely used in avian system to study development and diseases, but is not suitable for mammals which do not produce the retrovirus receptor TVA.

近年来,鸟类逆转录病毒载体(RCAS)及其受体(TVA)系统在哺乳动物转基因模型中得到广泛应用。

self-inactivating retroviral vector

自(动)失活逆转录病毒载体

Keywords bFGF;retroviral vector;gene transfer;

逆转录病毒载体;基因转移;

Recombinant retroviral expression vector

重组逆转录病毒载体

Keywords NT3;Retroviral Vector;DNA Recombinant;

关键词NT3;逆转录病毒载体;基因重组;

Packaging of Recombinant Retroviral Vectors in Vitro

重组逆转录病毒载体的包装

Immunity and Retroviral Superantigens in Humans, HMS Beagle

免疫性有毒病菌

pSiRNA-Notchl retroviral vector

pSiRNA-Notch1逆转录病毒载体

pSiRNA-Shh retroviral vector

pSiRNA-Shh逆转录病毒载体

Keywords p58 2 gene Retroviral vector Polymerase chain reaction;

2基因;逆转录病毒载体;聚合酶链式反应;

defective retroviral particle

缺陷性逆转录病毒颗粒

Keywords Stem cells;neurons;Retroviral;Transfection;Tumorigenicity;

干细胞;神经元;逆转录病毒;转染;致瘤性;

full-length retroviral sequence

全长逆转录病毒序列

Recombinant retroviral vector

重组逆转录病毒载体

Recombinant retroviral vectors

重组逆转录病毒载体

retroviral protease inhibitor

蛋白酶抑制剂

Keywords neural stem cell;retroviral;tumorigenicity;

关键词神经干细胞;逆转录病毒;致瘤性;

Preparation of the High Titer Retroviral Viruses for Transducting PDGF A Gene

供转导PDGF-A基因的高滴度逆转录病毒的制备

Keywords analgesia;proenkephalin gene;retroviral vector;APA microcapsule;

镇痛;前脑啡肽原基因;逆转录病毒载体;APA微囊;

Keywords Apoptin;Retroviral vector;Apoptosis;Hepatocellular carcinoma cell;

凋亡素;逆转录病毒载体;细胞凋亡;肝癌细胞;

Keywords Targeting retroviral vector;HDV ribozyme;Hepatitis B virus;

靶向性逆转录病毒载体;HDV核酶;乙型肝炎病毒;

Synchroization of cells significantly improves transduction efficiency of retroviral vector

细胞同步化能显著提高逆转录病毒载体的转染效率

Construction and Expression of Recombinant Retroviral Vector Containing D-Amino Acid Oxidase cDNA

D-氨基酸氧化酶基因重组逆转录病毒载体的构建及表达