trypsin
The second enzyme (0.25% trypsin) was used to dissociate RPE cells.
第二步(胰蛋白酶)用于离散RPE细胞,制成细胞悬液;
Mut-HPI was less easily digested by trypsin and CPB than Met-HPI.
Mut-HPI与改造前的Met-HPI相比，不易被胰酶和羧肽酶B(CPB)降解。
Two trypsin isoforms from the intestine of the grass carp.
草鱼与青鱼主要消化酶的活性研究。
The activity of bovine trypsin is less influenced by tween.
吐温对牛胰蛋白酶活性的影响较少。
The protein metabolic rate was relative to the trypsin activity in chyme.
不同鸡品种间蛋白质代谢率的差异与消化道胰蛋白酶活性高低相对应。
Objective To establish PCR assay of Porcine Parvovirus detection in Trypsin.
目的建立原材料胰蛋白酶中猪细小病毒的PCR检测方法。
Another proteolytic enzyme, trypsin, is quite inactive at that PH but functions effectively at a PH of 8.
另一种蛋白质水解酶--胰蛋白酶在此PH时活性甚低而在PH8时效率最高。
Either of two protein subunits of a myosin molecule, obtained especially through the digestive action of trypsin.
酶解肌球蛋白肌球蛋白分子的两种蛋白质结构单元的一种，主要通过胰蛋白酶的消化作用而获得
Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture.
方法：采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液，进行培养。
Either of two protein subunits of a myosin molecule,obtained especially through the digestive action of trypsin.
酶解肌球蛋白肌球蛋白分子的两种蛋白质结构单元的一种，主要通过胰蛋白酶的消化作用而获得
Epidermis was separated from dermis,cut into pieces, digested with 0.25% trypsin for about 5-7 mins.
分离表皮与真皮,将表皮剪成糜状,0.25%胰酶37℃消化5-7 min后中和,过滤,离心,接种。
Comparison of the methods shows that isolation of the OLC by trypsin can obtain the purest and greatest amount of OLC.
通过比较，结果显示：酶消化法提纯程度最高，收获量较多；
Perfusing the infrarenal abdominal aorta with trypsin developed canine AAA model.
用胰蛋白酶灌注犬肾下腹主动脉段形成动脉瘤模型；
Methods Dorsal root ganglions (DRG) from rat embryos were isolated under microscope and dissociated in trypsin.
方法取胚胎大鼠背根神经节,通过胰蛋白酶消化、抗有丝分裂药物纯化等方法获得背根神经节神经元。
Trypsin, the hydrdytic enzyme of insects, was crucial for digestion and toxicity of Cry protein.
Trypsin是昆虫体内存在的一种蛋白水解酶，它对Cry蛋白的作用结果关系到Cry蛋白毒性的发挥。
Overcoming trypsin denaturation and precipitation during AOT/Isooctane reverse micellar extraction by adding ethanol.
[10]陈霖杰.用乙醇消除AOT/异辛烷反胶束体系萃取过程中蛋白质的变性失活.华东理工大学硕士学位论文,2005Chen L J.
This paper has studied the extraction of collagen from porcine skin by trypsin, papain and pepsin.
研究了用胰酶、木瓜蛋白酶和胃蛋白酶从猪皮中提取胶原蛋白。
The serum in the medium in step 5 will inactivate the remaining trypsin and prevent cell damage.
在第 5 步骤的媒体的浆液将会使剩余的胰岛素钝化而且避免细胞损害。
In this paper,the enzymatic degradation of succinylated gelatin with trypsin was studied.
对以胰蛋白酶为催化剂,琥珀明胶为底物的酶解反应体系进行了考察。
The whole spinal cords of neonatal rats were dissociated and single cells were separated by trypsin digestion.
本研究探索利用新生大鼠脊髓培养和纯化少突胶质细胞的方法。
OBJECTIVE: To observe the therapeutic effects of urinastatin, trypsin inhibitor(UTI), on mild acute pancreatitis.
目的:观察乌司他丁治疗急性轻症胰腺炎的疗效。
Corn germ protein was hydrolyzed by trypsin and the effects of hydrolysates on mouse immunity were investigated.
用胰蛋白酶水解玉米胚芽蛋白,研究了玉米胚芽蛋白酶解物对小鼠体内的免疫调节作用。
When two enzymes are used,first trypsin then pepsin,the hydrolytic result is best.
用双酶水解时，先用胰蛋白酶后用胃蛋白酶，水解效果最好。
When the amount of papain reached optimal quantity,the activity of trypsin inhibitor would be steady.
木瓜蛋白酶的加入量达到最佳值后胰蛋白酶的活性趋于稳定而不再呈上升的趋势；
Under the microwave heating, alkling protease and trypsin were used to hydrolyze soy protein isolate in this paper.
研究微波加热条件下,用碱性蛋白酶和胰蛋白酶双酶水解大豆分离蛋白。
Methods: Blood samples of the patients were cultured and chromosome bands were showed using trypsin and Giemsa.
方法采用外周血淋巴细胞培养和G显带染色体核型分析。
In addition, a very low amount of trypsin is required and few trypsin autolysis peaks are observed.
其次我们详细介绍了目前蛋白质组学研究中的主要技术手段，一是经典的双向凝胶电泳技术和生物质谱技术，二是目前正在快速发展的生物样品多维色谱分离技术及众多杂交模式，这些技术的发展极大的提高了蛋白质组学研究的自动化水平，同时更有效的提高了蛋白的鉴定水平。
When the clones growed confluently 85% or above, they was digested with 0.25% trypsin and treated by 3-MC or TCDD.
待细胞生长汇合度达到85%以上，消化细胞，用3-MC或TCDD诱导筛选克隆。
Methods: Osteoblasts were isolated from neonatal r at calvaria through trypsin and collagenase digestion.
方法:酶消化法分离培养新生大鼠颅盖骨成骨细胞；
Methods HPMC was separated with trypsin and the synthesis of DNA was measured by 3H-TdR incorporation.
方法采用胰蛋白酶消化法，从人腹膜组织中分离间皮细胞，并建立体外人腹膜间皮细胞(HPMC)培养模型；
The two kinds of IgY could resist to the enzymolysis of pepsin, but were sensitivity to the trypsin.
两种抗体均可耐受胃蛋白酶作用，但对胰蛋白酶敏感。
Methods: The fetal RPE cells were cultured with trypsin enzyme digesting technique and passaged in vitro .
方法:用胰酶消化法获取人胎儿视网膜色素上皮细胞进行原代及传代培养。
Care should be taken to avoid leaving cells exposed to the trypsin longer than necessary.
照料应该被采取避免让细胞暴露到长于必需品的胰岛素。
An enzyme secreted by the upper intestinal mucosa that converts the inactive trypsinogen to the digestive enzyme trypsin.
肠激酶上肠粘膜分泌出的一种酶，将不活跃的胰蛋白酶原转化为消化性胰蛋白酶
To hydrolyze the sea cucumber collagen, four proteases are used as follows:3942 neutral proteinase, trypsin, papain, bromelain.
本实验选用3942中性蛋白酶、蛋白酶、瓜蛋白酶、萝蛋白酶四种酶对胶原蛋白进行水解。
The effects of trypsin at different dosage (0.0015%, 0.003%, 0.005%) on proteolysis of Chinese-bacon and flavor formation were studied.
对将胰蛋白酶以不同的添加量(0.0015%、0.003%、0.005%)添加到腊肉的生产工艺进行了研究。
Methods: Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture.
方法：采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液，进行培养。
Results In EDTA group, conjunctival fibroblasts grew very well. In trypsin group, fibroblasts in 6 of the 18 flasks did not grow well.
结果edta组兔结膜成纤维细胞生长良好,胰蛋白酶组18瓶中有6瓶生长不良。
This paper reports the application and research development of trypsin inhibitor in the field of medicine,plant disease resistance and feed industry.
对其在医药领域、植物抗病虫害、饲料工业方面的应用及有关的研究进展进行综述
Conclusion Lower concentration of trypsin combined with EDTA and scraping can effectively protect cell membrane structure and function.
较低浓度胰蛋白酶结合EDTA联合机械刮擦方法能较好地保持RPE细胞膜结构及其糖蛋白的功能状态。
One such protein is bovine pancreal trypsin inhibitor (BPTI), which has 58 amino acid residues in a single.chain and contains three disulfide bonds.
此种蛋白质的一个例子就是牛胰胰蛋白酶抑制剂(BPTI)，它有58个氨基酸残基，呈但链，含有三个二硫键。
The inactive precursor of trypsin,produced by the pancreas and converted to trypsin in the small intestine by enterokinase.
胰蛋白酶原胰朊酶的惰性前体;由胰制造并由肠激酶在小肠内转化为胰朊酶
One such protein is bovine pancreal trypsin inhibitor (BPTI), which has 58 amino acid residues in a single chain and contains three disulfide bonds.
此种蛋白质的一个例子就是牛胰胰蛋白酶抑制剂(BPTI)，它有58个氨基酸残基，呈但链，含有三个二硫键。
Antibiotic Trypsin Versen(ATV)combing with pipette is more appropriate to digestion and passage of SVEC compared with 0.25% trypsin.
与0.25%的胰蛋白酶相比，用ATV使用液结合吸管吹打更适合于猪血管内皮细胞消化传代。
Conclusions: Infant foreskins treated with dispase and trypsin and cultured with 254 containing HMGS, were suitable for melanocyte culture.
结论：小儿包皮用分离酶结合胰酶消化，以含HMGS的254培养基孵育可获得大量纯净的表皮黑素细胞。
In trial experiment endometrial lymphocyte(EML) from pregnant rabbit were obtained by digestion with trypsin and then by decenfugation.
预实验采用酶消化及密度梯度离心法分离妊娠家兔EML，并采用噻唑蓝（MTT）法进行体外活化试验。
Because of the speciality that keratinocytes stick to flask fast, we purified the keratinocytes by 0.02% EDTA and 0.05% trypsin step by step.
利用猪角朊细胞与培养瓶黏附牢固的特点,用0.02%乙二胺四乙酸(ethylenediaminetetraaceticacid,EDTA)和0.05%胰蛋白酶分步消化,纯化细胞。
Biological characteristics of Bt Cry proteinThe solubility, digestion of Cry protein with trypsin and growth speed of E.
Bt Cry蛋白特性的研究
Methods: The effect of conjugated bile acids on the rate of protein hydrolysis by trypsin and chymotrypsin was examined in itro.
方法：体外实验检测结合胆汁酸对胰蛋白酶和糜蛋白酶水解蛋白速率的影响。
Irreversibly inhibits trypsin but not chymotrypsin by alkylating the histidine residue in the active site of the enzyme.
通过乙酰化酶活性位点剩余的组氨酸，不可逆的抑制胰蛋白酶（但不含胰凝乳蛋白酶）。